1、中國農(nóng)業(yè)大學碩士學位論文表達OCI和SOS基因的轉(zhuǎn)基因甘薯植株的再生和鑒定姓名：張美彥申請學位級別：碩士專業(yè)：作物遺傳育種指導教師：劉慶昌20070501AbstractThetransfonntionwasstudiedbyusingembryogenicsuspensionculturesofsweetpotatocvsXushu18andLizbdangandAgrobacteriumtwnefaciensstrainEHAl05

2、harboringabinaryvectorpCAMBIAl301orpCAMBIA3301ThebinaryvectorpCAMBIAl301containsOryzacystatinIgene(卿，13glncuronidnsegene刪)andneomycinphosphotransferasegene(NPTII)ThebinaryvectorpCAMBIA3301containssaltoverlysensitivegenes

3、SOS(SOSlSOS2＆sos3)andbargeneThemainresultsaleasfollowsCellaggregatesfromembryogenicsuspensionculturesofXushu18werecoeultivatedfor3dayswiththeAtumefaciensstrainEHAl05harboringabinaryvectorpCAMBIAl301onthesolidMSmediumwith

4、20mg／L24Dand30mg／LASTheresultsindicatedthatthefrequencyofcellaggregatesexpressingtransientGUSactivityreached9267％The1560inoculatedcellaggregateswerefirstculturedonMSmediumcontaining20mg，L2，4Dand100mg／LCarbfor1weekandthen

5、culturedontheselectionmediumwith20mg／L2，4D’100mg，LCarband7mg，LHygandformed133HygresistantcallusesAftertransferredtosolidMSmediumwith10mgCtABAandlOOmg／LCarbtheyregenerated552plantsGUSassayofthe30regeneratedplantsrandomlys

6、ampledshowedthat10ofthem(3333％1expressedGUSactivityPCRandSouthernblotanalysesofthe24regeneratedplantsrandomlysampleddemonstratedthat8ofthem(33％)weretransgenicplantsCellaggregatesfromembryogenicsuspensionculturesofLizixia

7、ngwerecocultivatedfor3dayswiththeAtumefaciensstrainEHAl05harboringabinaryvectorpCAMBIA3301onthesolidMSmediumwith20mg／L24Dand30mg／LASAfterculturedoiltheselectionmediumwith20mg／t2，4D，100mg／LCarband05mgCtPPT’the320inoculate

8、dcellaggregatesproduced3PPTresistantcallusesThePPTresistantcallusesweretransferredtoMSmediumwith10mg／LABAand100mg／LCarbandregenerated7plantsPCRassayshowedthat4ofthe7regeneratedplants(58％)weretransgenicplantsKeywords：Swee