1、中國協(xié)和醫(yī)科大學(xué)碩士學(xué)位論文急性白血病MLL基因重排的檢測(cè)姓名：趙佳煒申請(qǐng)學(xué)位級(jí)別：碩士專業(yè)：病理學(xué)和病理生理學(xué)指導(dǎo)教師：王敏20070501中國醫(yī)學(xué)科學(xué)院中國協(xié)和醫(yī)科大學(xué)碩士學(xué)位論文DetectionofMLLGeneRearrangementsinPatientswithAcuteLeukemiaAbstractRearrangementsofMLLgeneoftenOCCUTFcdinacuteleukemiaespecially

2、treatedwiththeinhibitorsofmpolIMLLgenelocatedat11q23anditisdifficultbyconventionalcytogeneticanalysistouncovertheabnormal1lq23SoisFISHmethod，whichistimeandlaborgOllSUnledandhardtodetecttheminimalresidualleukemiccellswith

3、abnormal1lq23ThusweplantosetupaconvenientandrapidmethodtodetectMLLgenearrangementsinpatientswithleukemiaThenthedetectionresultinturnwouldcontributetotheclinicaldiagnosisofleukemiaandtherapy—relatedMLLgenearrangementsWees

4、tablishedtwotypesofpanhandlePCRmethods，panhandlePCRandreversepanhandlePCRtodiagnosetherearrangementsofMLLgenePanhandlePCRandreversepanhandlePCRmethodsareobviouslybetterthantheconventionaldetectionmethods，such船cytogenetic

5、analysis，F(xiàn)ISHexperiments，andspecificRT—PCRmethodsTheyarebothuniveralmethodswithhighsensitivity，easytoapplication，andtheyrequireonlysmallquantitiesofgenomicDNAAndwhat’smorebothofthemcouldidentifyeachchromosomalbreakpoints

6、itesandobtainmoredetailedinformationThenthoseinformationcouldcontributetofurtherunderstandthemolecularmechanismofMLLarrangementsandtherelationshipamongthesubtypeofMLLarrangements，therapeuticregimens，andclinicalprognosis1

7、11eacuteleukemiapatientstreatedwiththetopolIinhibitotsandthedenovoacuteleukemiapatientswereusedinourstudy，andthepatientwitht(4；11)aspositivecontr01WesuccessfullyampplifiedtheabnormalbandbypanhandlePCRandreversepanhandleP