1、河北大學(xué)碩士學(xué)位論文GMAST共聚物的制備及其固定化米曲霉β半乳糖苷酶的研究姓名：徐小冰申請學(xué)位級別：碩士專業(yè)：物理化學(xué)指導(dǎo)教師：孫素芳201106AbstractIIAbstractTwokindsofmacropouspoly(GMAST)weresynthesizedbybulkcopolymerizationusingdifferentpefmingagentsoneonlywithliquidpogenicagentinclu

2、dingcyclohexanollaurylalcoholtheothersimultaneouslywithamixtureofcylohexanollaurylalcoholnanocalciumcarbonate.Afterthepolymerwassmashedparticleswithdiametersranging0.15mmto0.30mmweretakenasthecarrier.Scanningelectronmicr

3、oscopy(SEM)micrographsweredonetoacteritssurfacestructure.Undertheoptimumconditionsβgalactosidasewasimmobilizedonthecarrierobtainedabovetheenzymeactivitytheactivityyieldthebasicpropertiesoftheimmobilizedenzymeweredetermin

4、edseparatelysatisfactyresultswereobtainedinenzymeactivityactivityyieldpHstabilitythermalstabilityoperationalstability.Theconclusionobtainedindicatedthattheactivityoftheimmobilizedenzymeontheploy(GMAST)preparedconcurrentl

5、ywithliquidsolidpogenreachedamaximumof535.11Ugdrycarrier.Soitwasmesuitabletoimmobilizeenzymethanthatpurelywithliquidsolutionaspefmingagents.Macropouspoly(GMAST)particlesweresynthesizedviasuspensionpolymerizationwithamixt

6、ureofcyclohexanollaurylalcoholnanocalciumcarbonateaspefmingagents.SEMmicrographswerealsodonetoacteritssurfacestructure.Undertheoptimumconditionsβgalactosidasewasimmobilizedonthecarrierobtainedabovethepropertiesoftheimmob

7、ilizedβgalactosidaseweredetermined.Theresultshowedthatthecarrierobtainedbysuspensionpolymerizationwasmesuitabletoimmobilizeenzymethanthatobtainedbybulkcopolymerization.Novelmagicbeadswerepreparedfromglycidylmethacrylates

8、tyreneviasuspensionpolymerizationwithcyclohexanollaurylalcoholaspogenicagent.Themagicpoly(GMAST)beadswereacterizedwithSEMmagicscalesXrayspectroscopy.Themagicsusceptibilityofthemagicpoly(GMAST)beadswas1.25104cm3g.Underthe

9、optimumconditionstheβgalactosidasewasimmobilizedonthemagicpoly(GMAST)beads.Theactivityoftheimmobilizedβgalactosidasereached412.09Ug.Itwasmesuitabletoimmobilizeenzymethannonmagiccarrier.Howeverthebasicpropertiesoftheimmob