1、遼寧師范大學碩士學位論文靈芝硒多糖對乳腺癌MCF7細胞蛋白質組的影響姓名：王丹申請學位級別：碩士專業(yè)：細胞生物學指導教師：尚德靜20090501靈芝硒多糖對乳腺癌細胞MCF7蛋白表達的影響的研究Abstract：Seleniumisallessentialtraceelementforhumanbeingsaswellasplaysallimportanteffectonanti—cancer，anti—aging，improvingi

2、mmunitypreventionandtreatmentofcardiovasculardiseaseandKeshandiseaseThereisconsensusthatorganicSehashigherbioavailabilityandlowertoxicitythaninorganicspecies，andisconsideredtobesaferOrganicseleniumincludesSecontainingpol

3、ysaccharides，Se—containingproteinsandSOon，ofwhichSecontainingpolysaccharidesexhibitnotonlySeactivitybutalsoavarietyofphysiologicalfunctionsofpolysaccharideInorganicSeCanbebiotransformedintoorganicformsbyediblemushroomins

4、ubmergedSe—enrichedcultureofediblemushroomAndGanodermalucidumexhibitsthehighestbiotransformactivityResearchshowsthatSe—containingpolysaccharidefromSeenrichedG1ucidumisabroadspectrumimmuneenhancerwi也anti—cancer，anti—aging

5、，anti—infective，antiradiationandotherphysiologicalfunctionsAtpresent，theresearchesonanticancermechanismofSe—containingpolysaccharidefocusontheeffectsofsingleproteinorsomepathwayButtheimpactsofSe—containingpolysaccharideo

6、ncancercellsaremultifactors，andinevitablyinvolvemanymetabolizedpathwaysInaddition，theinteractionoccuringastwoormoreproteinsisdiverse，dynamicandresultesinaseriesofchangesinexpressionofproteinsTherefore，itisnecessarytomake

7、studyonawhole，systemiclevelFirstofall，theSe—containingpolysaccharideSeGLP一2B一1wasisolatedandpurifiedfromtheSe—enrichedmyceliaofG1ucidumThenvariousconcentrationsofSeGLP一2B一1wasaddedtoMCF一7cellsandincubatedfor20h，44h，68h，a

8、ndcellviabilitywasassessedbytheMTTmethodTheresultshowedthattheMCF一7cellstreatedwithSeGLP2B1begantoapoptosis，andtheapoptosisratewas2589％afterculturedfor24hThehighestinhibitionratecouldreachto83％afterculturefor72h，andIC50w

9、asO158ItMItmeansthatSecontainingpolysaccharidefromG1ucidumhasanti—tumoractivityUsingthetechnologyofComparativeproteomics，thedifferenceinexpressionofproteinsWasinvestigatedbetweenMCF7cellstreatedwithSeGLP2B1for48handcontr

10、olgroupbytwo—dimensionalgelelectrophoresisTheresultsshowedthattheexpressionofproteinsexhibitedmuchmoredifferencesinbetweenthecontrolgroupandexperimentalgroup5differentspotswerecutfromthegelsandwerecarriedoutanalysisusing

11、MALDI—TOFMS／MSafteranalyzatedbyPDQuestsoftwareThefiveproteinswereAnnexinA2，keratin8，tropomyosin3，heatshockprotein90andtyrosine3monooxygenase／tryptophan5monooxygenaseactivationprotein，respectivelyThepreviousstudiesprovedt