1、y956580蠶。鑭戈誓碩士學(xué)位論文(2006屆)鼠ING4基因腺病毒表連載體的構(gòu)建及抗腫瘤效應(yīng)的實驗研究TheexperimentalstudyonrecombinantexpressionandantitumoreffectofING4研究生姓名至盎越指導(dǎo)教師姓名———舅島叢量—揚盜直—一專業(yè)名稱盅蕉塋研究方向塑』g墨墅生論文提交日期————生迦生生立且——一Theexperimentalstudyonrecombinantexpr

2、essionandantitumoreffectofmlNG4Abstractobjective：ToconstructtherecombinedadenovinesvectorofruING4andexploretheeffectofING4onmSMMC7721cellsMethods：ThepAdTrackCMVmlNG4wasconstructedwi廿1pcDAN30一mlNG4recombinedplasmidbyPCRas

3、template，enzymedigestionandligationThepAdTrackCMVmlNG4linearedbyPmelwasco—transductedintoBJ5183withpAdeasy—IAnalysisofPCRandDNAsequencingwascarriedouttodemons仃atethesequenceoftheplasmidThepAdeasy1一pAdTrackCMVmlNG4recombi

4、nedadenovirusvectorwaslearedwitllPacIandthentransfectedintoQBI一293AcellsThemlNG4recombinedadenovimswasobtainedandwasusedtoinfectS刪C7721cellsIdentificationofmlNG4mRNAbyRTPCRwascarriedouttodemonstratetheexpressionofmlNG4in

5、SMMC7721cellsTheeffectofruING4onSMMC7721wasstudiedbyMrTflowcytometryandwestemblotResults：AnalysisbyrestrictingenzymedigestionandPCRofpAdTrack—CMVmING4recombiantplasmidshowedresultswereabout750bp，DNAsequencingrevealedthat

6、mING4cloningweresuccessfulCellcycleandapoptosiswereexaminedbyflowcytometryafterinfectionofSMMC7721cellswithpAdeasy一1一pAdTrack—CMVmlNG4(AdmlNG4)TheinhibitionofmlNG4ongrowthofSMMC7721cellswastestedbyMTTWefoundthatmlNG4coul