1、華中農(nóng)業(yè)大學(xué)碩士學(xué)位論文茯苓菌絲體培養(yǎng)條件及其富集鈣和碘的研究姓名：王偉霞申請學(xué)位級別：碩士專業(yè)：微生物學(xué)指導(dǎo)教師：陳立國20030501ABSTRACTBeingtheoneoftraditionalChinesemedicine，WolfiporiaCOCOSisakindofedineandmedicinalfungiwhichhasgreatvaluetostudyWhenthemyceliasecretedmelanin，th

2、egrowthofmyceliawasinhibitedThispaperstudiedtheconditionofsolidcultivationandliquidfermentation；Andthispaperstudiedtheconditionofcalcium。enrichedandiodineenrichedofWo班poriaCOCOSTheresultsofthestudywereasfollows：1Nutritio

3、ntestsofWotfiporiaCOCOSsuggestedthattheoptimalcarbonsonlcc，nitrogensource，mineral，growthfactorpH，C／Nvaluewerepinewoodandglucose，cornplasm，KH2P04andMgS04，VBI，317～605，25：1～35：1respectivelyAndsecretingmelaninofthemyceliadid

4、notrelatetocarbonsource，mineral，growthfactorandC／NWhennitrogensourcewereorganicnitrogenwhichdidn’tincludecornplasm，theconcentrationofKIWashigh，orpHWasgreaterthan70，themyceliaofWolfiporiaCOCOSsecretedmelanin2Temperaturete

5、stsuggestedthatwhentheWolfiporiaCOCOSwhichcultured22℃waspreservedafter7days，andwhichcultured28“CWaspreservedafter9days，themyceliabegantosecretmelaninButthemyceliawhichcultured25‘Conlybegantosecretmelaninafter30days3Thest

6、rainofWotfiporiaCOCOSisolatedfernpultaceoussclerotiumsecretedthemoremelaninthanthatofthestrainisolatedfromrigidsclerotiumCulturingmycelia’StipofWolfiporiaCOCOScouldnotinhibitthatthemyceliasecretedmelaninwell4Inoculationn

7、umberandmediumvolumetestssuggestedthattheoptimalinoculationnumberwas10％，andtheoptimalmediumvolumeWas50mL／150mL砸thErtenmeyerflask。ThroughorthogonaltesttheoptimalmediumWaSselectedanditcontainedglucose259，COITIplasm10mL，KH2

8、P04159ldgSOO759VB5mg，distillationwater1000mLAndthismediumWasbetterthanCOrnplasmmediumtoWolfiporiaCOCOSUndertheoptimalfermentationconditionwegotthevariationcurvesofmyceliabiomass，pHvalue，extracellularpolysaccharideandendo

9、cellularpolysaccharidecontent5ThetestofenduringcalciuminthesolidmediumsuggestedthatthemyceliagrowthwasnotinhibitedwhentheconcentrationofCaCl2Was200eeLWhentheconcentrationofCaCIzWaS4009／Lorgreaterthanitthemyceliagrowthwas

10、inhibitedWhentheconcentrationofCaCl2Was2009／Landaddedwithconcentrationgrads，thetestofcalciumenrichedinthecourseoffermentationsuggestedthatenrichingratiowas7846％，calciumcontentofthemyceliaWas9425mg／g，and2683％wasorganiccal